Phenotypic identification of bacteria by biochemical tests (fermentation, substrate utilization etc) is still relevant.
I have developed software applications that will enable users to identify the organisms based on the results of their tests.
The software uses probability matrix for identification and the results are expressed in percentage probabilities.
The matrix table can also be used to view the properties of these organisms and to compare their properties.
Accuracy of the results are dependent on the accuracy of the test results. Please note that the matrices used here have not been updated since more than a decade.
Databases used here have been created from the matrices/tables published elsewhere. Identification of gram positive cocci, aerobic gram negative bacilli, coryneforms, Bacillus spp, rapidly growing Mycobacteria, Aeromonas etc would be made available in due course of time.
This method uses 16 physiological tests to identify Enterobacteriaceae members.