Peptone water, Nutrient broth and agar.
Blood agar, Chocolate agar, Dorset egg medium and Loeffler’s serum medium.
Amies medium, Pikes medium, Stuart’s medium, Buffered glycerol saline and VR medium.
Selenite F broth, Tetrathionate broth and Alkaline peptone water.
Most media are sterilized by autoclaving.
By adding 2 - 3% agar.
5% defibrinated sheep blood.
95°C and 45°C respectively.
By adding 5% blood to hot, melted nutrient agar. It is also known as lysed blood agar.
Loeffler’s serum slope and Hiss serum water.
Dorset egg medium and LJ medium.
Milk agar and glycerol monoacetate agar.
24°C. It remains as liquid when incubated at 37°C.
Colistin-Nalidixic acid Agar (CNA).
Robertson’s Cooked Meat medium (R.C.M), chopped medat glucose broth and Thioglycollate broth are the liquid media. Blood agar with suitable blood agar base, reducing agents and nutrition supplements can be used to grow anaerobes.
Glucose broth, Bile broth and Brain heart infusion broth.
Agar is required in smaller quantity (2-3%, in contrast to 10-15% of gelatin), is solid at the incubating temperature (while gelatin is liquid at this temperature) and does not contribute to the nutritional property of the medium (gelatin is liquefied by some bacteria).
Indicator media indicates the presence of certain bacteria by the change in colour. MacConkey's medium indicates the growth of lactose fermenters by pink coloured colonies.
Media that aid in differentiation among bacteria based on some phenotypic property (such as sugar fermentation) are differential media. MacConkey differentiates LF from NLF.
Media that are supplemented with extra nutrition in the form of blood, serum, egg yolk etc are enriched media. They are suitable for fastidious bacteria.
Biphasic medium contains both solid and liquid medium in the same bottle. Blood is inoculated into the liquid medium and subcultures are performed by tilting the bottle. Used in Castaneda method of Blood culture.